Friday, November 13, 2020

Real Time PCR Testing In Canada: Cycle Thresholds and Grossly Innacurate Test Results- Take the REAL Science to the Politicos

We are the only ones that will put a stop to this madness! Save this information for yourself. Pass it on to others. Hold public officials accountable for this psychological operation.

Continuing on from previous report regarding the distorted results with the PCR tests.

PCR "Pandemic" Testing- Please Raise This Issue With Your Public Politicos Including Health Officers-

"compared with less than 3% of the cases with CT values above 35" (able to be cultured)

Let's talk about that? Less then 3 percent of the cases with CT values above 35 could be cultured. Contained live virus that would multiply, grow or spread. I will be posting the CT values from Canadian provinces. Do you think any of them are below 25 cycles? If you do? Think again! 

 Less then 3 percent of cases in the cited study would result in anyone being sick, let alone making any one else sick. No ability to grow anything (culture) Therefore there would be no pandemic without high CT values.

What do you suppose the numbers are here in Canada. Province, by province.

Look at the high CT values- the lowest is 30 the highest is 42. If LESS THEN 3 percent of positive tests with a CT value above 35 could be cultured what do you suppose are the REAL numbers of people who are true Covid cases versus contrived positive test results?

 Below is the data:

 Brief Intro: Real-time PCR-based SARS-CoV-2 detection in Canadian laboratories

1. Introduction

Coronaviruses are part of a large family of viruses that infect humans and animals, and are classified into 4 genera (α, β, γ, and δ). Human coronaviruses belong to α and β genera. [1] Most human coronaviruses (229E, NL63, OC43, and HKU1) are causes of the common cold, and serious outcomes are rare. Some human β-coronaviruses have been associated with high morbidity and mortality, such as severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle East respiratory syndrome coronavirus (MERS-CoV)].......

2. Methods

The lower limit of detection (LoD) of each method was determined by testing serial dilutions of RNA extracted from a cultured stock of SARS-CoV-2/Canada/VIDO-01/2020 [originally cultured by VIDO-Intervac at the University of Saskatchewan from a clinical specimen originating from Sunnybrook Health Sciences Centre, and propagated at the National Microbiology Laboratory (NML)].........

 

LaboratoryThermocyclerRT-PCR reagentsReaction vol. (μL)Template vol. (μL)* Positivity cutoff (Ct value)SARS-CoV-2 TargetLoD (log copies/mL; 95% CI)
AB (ProvLab)ABI 7500 Fast (Applied Biosystems)TaqMan Fast Virus-1-Step Master Mix (Life Technologies)201035RdRp4.243 (3.472−5.013)
E3.776 (3.122−4.430)
Any33.776 (3.122−4.430)
BC (BCCDC)ABI 7500 Fast (Applied Biosystems)TaqMan Fast Virus-1-Step Master Mix (Life Technologies)20535RdRp3.591 (3.245−3.937)
E3.579 (3.180−3.979)
Any33.579 (3.180−3.979)
BC(BCCW)ABI 7500 Fast (Applied Biosystems)TaqMan Fast Virus-1-Step Master Mix (Life Technologies)20535RdRp4.243 (3.472−5.013)
E4.729 (3.683−5.774)
Any33.901 (3.048−4.755)
BC (SPH)LightCycler 480 (Roche Diagnostics)LightMix 2019-nCoV Real-time RT-PCR kit (Tib Molbiol) with Lightcycler Multiplex RNA Master (Roche Diagnostics)20540E3.901 (3.048−4.755)
BC (VGH)ABI 7500 Fast (Applied Biosystems)TaqMan Fast Virus-1-Step Master Mix (Life Technologies)20535RdRp4.516 (3.677−5.354)
E4.516 (3.677−5.354)
Any34.243 (3.472−5.013)
BC (VIHA)ABI 7500 Fast (Applied Biosystems)TaqMan Fast Virus-1-Step Master Mix (Life Technologies)20535RdRp4.375 (3.413−5.337)
E3.901 (3.048−4.755)
Any33.809 (2.886−4.732)
MB (Cadham)CFX96TM (Bio-Rad Laboratories, Ltd.)TaqMan Fast Virus-1-Step Master Mix (Life Technologies)20536.5E3.901 (3.048−4.755)
N13.776 (3.122−4.430)
N23.632 (2.670−4.594)
N33.901 (3.048−4.755)
NB (CHU-Dumont)LightCycler 480 II (Roche Diagnostics)Lightcycler Multiplex RNA Master (Roche Diagnostics)20540E + N3.776 (3.122−4.430)
NLLightCycler 480 II (Roche Diagnostics)Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs)20533E + N23.575 (3.141−4.009)
NS (NSHA)ABI 7500 Fast (Applied Biosystems)TaqMan Fast Virus-1-Step Master Mix (Life Technologies)20535E3.901 (3.048−4.755)
RdRp3.776 (3.122−4.430)
Any33.901 (3.048−4.755)
ON (PHOL)Quantstudio 5 (ThermoFisher Scientific)TaqPath 1-Step Multiplex Master Mix (Applied Biosystems)10538E3.715 (3.114−4.316)
RdRp4.292 (3.658−4.925)
Any33.437 (2.829−4.045)
PE (QEH)BD Max (Becton Dickinson and Company)RealStar SARS-CoV-2 RT-PCR kit (Altona Diagnostics)2012.5ND1E3.901 (3.048−4.755)
S4.558 (3.498−5.618)
Any33.809 (2.886−4.732)
QCQuantStudio3 (ThermoFisher Scientific)TaqPath 1-Step Multiplex Master Mix (Applied Biosystems)20537E3.776 (3.122−4.430)
N4.234 (3.101−5.367)
SK (RRPL)ABI 7500 Fast (Applied Biosystems)TaqMan Fast Virus-1-Step Master Mix (Life Technologies)20536E3.752 (3.237−4.266)
RdRp3.566 (3.090−4.420)
Any33.078 (2.539−3.617)
ON (St-Joseph's Healthcare)CFX96TM (Bio-Rad Laboratories, Ltd.)Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs)20545UTR3.809 (2.886−4.732)
UTR + E2.638 (2.068−3.209)
ON (St-Joseph's Healthcare)Rotor-Gene Q (QIAGEN Inc.)Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs)20545UTR3.878 (2.685−5.071)
UTR + E3.632 (2.670−4.594)
Any33.878 (2.685−5.071)
ON (St. Michael's Hospital)Quantstudio 5
(ThermoFisher Scientific)
RealStar SARS-CoV-2 RT-PCR kit (Altona Diagnostics)3010ND1E3.375 (2.796−3.954)
S3.493 (2.951−4.034)
Any33.078 (2.539−3.617)
ON (Mt-Sinai)CFX96TM (Bio-Rad Laboratories, Ltd.)Allplex 2019-nCoV Assay (Seegene Inc.)25840E3.632 (2.670−4.594)
RdRp3.809 (2.886−4.732)
N3.632 (2.670−4.594)
Any32.638 (2.068−3.209)
ABI 7500 Fast (Applied Biosystems)Taqpath RT-PCR COVID-19 Kit with TaqPath 1-Step Multiplex Master Mix (Applied Biosystems)205ND1Orf1a/b4.243 (3.472−5.013)
N4.434 (4.002−4.866)
S4.434 (4.002−4.866)
Any33.776 (3.122−4.430)
ABI 7500 Fast (Applied Biosystems)RIDA GENE SARS-CoV-2 (r-Biopharm AG)205ND1E3.901 (3.048−4.755)
ABI 7500 Fast (Applied Biosystems)Lyra SARS-CoV-2 assay (Quidel Corp.)155302Orf1a/b4.712 (3.837−5.587)
ABI 7500 Fast (Applied Biosystems)DiaPlexQ Novel Coronavirus (2019-nCoV) Detection Kit (SolGent Co., Ltd)20540N3.901 3.048−4.755)
Orf1a3.809 (2.886−4.732)
Any33.809 (2.886−4.732)
ABI 7500 Fast (Applied Biosystems) in Standard modeNew Coronavirus Nucleic Acid Detection Kit (Perkin–Elmer)204042N4.516 (3.677−5.354)
Orf1ab3.776 (3.122−4.430)
N/AAny33.776 (3.122−4.430)
LighCycler 2.0
(Roche Diagnostics)
LightMix 2019-nCoV Real-time RT-PCR kit (Tib Molbiol) with Lightcycler Multiplex RNA Master (Roche Diagnostics)201036E3.632 (2.670−4.594)
39RdRp5.591 (4.769−6.412)
37N5.523 (4.636−6.410)
N/AAny33.632 (2.670−4.594)

From earlier today:

Nagorno Karabakh: “Russia , Turkey Have Common Objectives in Karabakh” as MOU signed

9 comments:

  1. Not to worry, Penny. There's a vaccine now. (sarcasm)

    I posted this yesterday, and I hope people will see the implications:
    - The first effective coronavirus vaccine can prevent more than 90% of people from getting Covid-19 ...

    -- According to the CDC, COVID-19 has thus far played a role in the deaths of just 0.05 percent of the U.S. population and was the sole factor in the deaths of a mere 0.003 percent.

    Do the math, eh? The vaccine has no chance of being unsuccessful. It could be just sterilized water and it would work!

    ReplyDelete
    Replies
    1. Hi Yaya

      such a busy day today, I'm going to try and stop over there and read your latest then get to GC's as well.

      Delete
  2. What is the best way to determine which tests are being used in various counties. Is this simply a matter of calling up various county health departments?

    ReplyDelete
    Replies
    1. Perhaps you could start with local or regional health departments ( we have regional health departments here in Canada) Call or check their websites?

      Delete
    2. If I come across anything relating to the US I'll post it here.

      Delete
  3. Hi Penny: Hope you're having a more or less restful weekend. Just came across this and thought it might interest you if you haven't already seen it:

    Why You May Not Be Able to Get Pfizer's Frontrunner COVID-19 Vaccine

    [It needs to be kept super cold and most places don't have fridges that go to the temperature needed.]

    People without access to these facilities, such as those living in rural areas, nursing homes and developing nations, may have to wait for other vaccines working their way through the development pipeline.
    https://time.com/5911543/pfizer-vaccine-cold-storage/

    (I don't know; maybe they've forgotten about the dead of winter???? I just hope they never solve that problem, because as is, it will be off the menu for most of the elderly, just the way I like my vaccines.)

    ReplyDelete
    Replies
    1. The whole Pfizer, stock market CEO shares sale, was such an obvious way to grab money- some caring government will buy the vaccines even if they destroy them. Can't starve big pharma. ever. Grrr...

      Delete
  4. Good stuff you have there Penny It is no wonder, why case numbers are so high.
    Del did a video about the Pfizer vaccine. I says no thanks to that one too. https://shadowsbearsoutlook.blogspot.com/2020/11/the-truth-about-pfizer-caccine-and.html

    ReplyDelete
    Replies
    1. The high CT's virtually guarantee a positive test result that is of no consequence

      I'll have a wach of Del's video- thanks

      Delete

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